WebDec 27, 2024 · (D and E) Quantification of BRN3A + RGC numbers (D) and cell survival rate (E) in aged Spp1 GFPfl/fl GfapCre mice, suggesting that astrocytic SPP1 deficiency accelerated age-dependent RGC loss (n = 7). (F) BRN3A-labeled RGCs in Spp1 GFPfl/fl and Spp1 GFPfl/fl GfapCre mice after high IOP. WebOct 8, 2013 · It is crucial to understand whether Brn3a AP RGCs loss seen in Brn3a KO, Brn3b KO or Brn3a–3b DKO retinas is a result of reduced RGC numbers, silencing of …
Frontiers Generation of a Transplantable Population …
WebBRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T … WebSep 21, 2024 · Brn3a requirement in early versus late RGC development. Brn3a is required for the development of ON and OFF β RGCs and some bistratified RGC types, and Brn3a KO animals have a net RGC loss of about 30% [5, 7, 85, 93]. Ablating either Brn3a, Brn3b or both in adult mice does not affect RGC numbers up to six months post ablation . fsb mary ann
Optimized fluorescent activated cell sorting (FACS)-based cell ...
WebIn a transforming growth factor-β model of glaucoma, we found that Brn3a + RGC numbers decreased by 33% whereas RNFL thickness had reduced by 51%(Mead et al., 2016). It … WebSep 21, 2024 · Background: While the transcriptional code governing retinal ganglion cell (RGC) type specification begins to be understood, its interplay with neurotrophic signaling is largely unexplored. In mice, the transcription factor Brn3a/Pou4f1 is expressed in most RGCs, and is required for the specification of RGCs with small dendritic arbors. WebApr 15, 2014 · In mouse and rat retina, most RBPMS cells are lost following optic nerve crush or transection at 3 weeks, and all Brn3a-, SMI-32-, and melanopsin … gift of jesus christ