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Cisplatin zebrafish

WebOct 21, 2024 · The zebrafish larvae model offers two significant advantages: (i) it can be used during a screening of novel substances and compounds against cisplatin-induced hair cell damage; (ii) it can promote a better understanding of the mechanisms related to cisplatin-induced hearing loss. 21 However, studies using zebrafish are not free of … WebMay 1, 2005 · Cisplatin causes changes in the zebrafish pronephric tubule typical of those seen in mammalian kidneys. After injection of cisplatin (4.6 nl, concentration 1.5 mg/ml) at 48 hpf, zebrafish with mild ( B ) or severe ( C ) decreases in inulin clearance can be distinguished by the level of residual fluorescence at 96 hpf, 24 h after the injection ...

Protective effects of apocynin on cisplatin-induced ... - PubMed

WebJan 1, 2024 · This study provides evidence that cisplatin-induced hair cell loss in zebrafish is accompanied by protein nitration and Lmo4 degradation, which are emerging as … WebJul 3, 2024 · Cisplatin is a widely used chemotherapeutic agent with a high degree of ototoxicity and an average incidence of over 60% ( Karasawa and Steyger, 2015 ). Ototoxicity depends on several factors, including patient … first oriental market winter haven menu https://sac1st.com

Functional mechanotransduction is required for cisplatin …

WebSep 1, 2024 · Zebrafish embryos were exposed to cisplatin (0, 50, and 100 μM) for 96 h (4–100 h post-fertilization) and then stained with fluorescent dyes to reveal mitochondrial activity (rhodamine123), apoptosis (acridine orange), and oxidative stress (CellROX/MitoSOX) in ionocytes of living embryos. WebFeb 18, 2024 · For this study, adult female zebrafish were given cisplatin (G2), the probiotic, Bacillus megaterium (G3), and cisplatin+ B. megaterium (G4) for 30 days, in addition to the control (G1). In order to investigate changes in antioxidative enzymes, ROS production, and histological changes after treatment, the intestines and ovaries were … WebMay 1, 2024 · Cisplatin is a well-known chemotherapy medication used to treat numerous cancers. However, treatment with cisplatin in cancer therapy has major side effects, such as nephrotoxic acute kidney injury. Adult vertebrate kidneys are commonly used as models of cisplatin-induced nephrotoxic acute kidney injury. first osage baptist church

Use of zebrafish larvae lateral line to study protection against ...

Category:Caspase 3/GSDME-dependent pyroptosis contributes to ... - Nature

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Cisplatin zebrafish

Cisplatin exposure acutely disrupts mitochondrial bioenergetics in …

WebFeb 15, 2024 · The cisplatin was used to produce a loss of CHCs to create a murine model of deafness. The AAV vectors were delivered into the scala media through the lateral wall. Compared with the control mice, the cisplatin-treated mice exhibited significantly enhanced apoptosis and autophagy in the cochleae, accompanied by a notably decreased Pou4f3 … WebMar 3, 2013 · Zebrafish were pretreated with cimetidine for 1 h, followed by cotreatment with 50 μ m cisplatin for an additional 24 h. We found no significant protection against cisplatin-induced hair cell loss for all concentrations of cimetidine evaluated ( p > 0.05, one-way ANOVA; Fig. 1a ). Figure 1.

Cisplatin zebrafish

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WebCisplatin is a very effective anticancer drug and generates reactive oxygen species (ROS) such as superoxide anions that can deplete antioxidant protective molecules in the cochlea. ... An in vivo study of transgenic zebrafish (Brn3C: EGFP) was used to investigate the protective effects of apocynin on cisplatin-induced hair cell death. In an in ... WebNational Center for Biotechnology Information

WebDec 1, 2024 · Zebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but … WebOur findings demonstrate that cisplatin can alter the locomotion behavior of zebrafish. Behavioral changes of zebrafish according to cisplatin-induced toxicity of the balance …

WebUsing zebrafish embryos as a model, cisplatin was previously found to impair skin ionocytes and ion regulation. The purpose of this study was to further investigate how cisplatin damages ionocytes. Zebrafish embryos were exposed to cisplatin (0, 50, and 100 μM) for 96 h (4-100 h post-fertilization) and then stained with fluorescent dyes to ... WebFeb 8, 2024 · Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi). In this model, …

WebNov 18, 2024 · Cisplatin is an effective anticancer agent, but also causes permanent hearing loss by damaging hair cells—the sensory receptors essential for hearing. There is an urgent clinical need to protect cochlear hair cells in …

WebZebrafish may offer key insights to cisplatin ototoxicity because their lateral-line organ contains hair cells that are remarkably similar to those within the cochlea but are optically accessible, permitting observation of cisplatin injury in live intact hair cells. first original 13 statesWebJul 7, 2024 · Using cisplatin to model CICI (), we performed RNA sequencing in the mouse hippocampus to identify novel molecular contributors and found profound up-regulation of the adenosine A 2A receptor (A 2A R) by cisplatin (Fig. 1A), potentially representing a mechanism for cisplatin-induced neurotoxicity.Adenosine maintains central nervous … firstorlando.com music leadershipWebCisplatin, an inorganic platinum derivative, is one of the most effective anticancer drugs and is widely used for the treatment of many malignancies, including brain, head and neck, lung, breast, and ovarian cancers [1][2]. Cisplatin acts by crosslinking purine bases in DNA. first orlando baptistWebMay 15, 2024 · The zebrafish has appeared as an interesting model to study renal function, kidney regeneration, and injury, as renal structures conserve functional similarities with mammals. Adult zebrafish injected with cisplatin shows decreased survival, kidney cell death, and increased inflammation markers after 24 h post-injection (hpi). firstorlando.comWebFeb 6, 2014 · Zebrafish were exposed to 1000 μM cisplatin for 6 h. We, then, periodically monitored swimming depth, total swimming distance, peak swimming velocity, and mean swimming velocity of the fish for approximately 21 days. first or the firstWebFeb 1, 2013 · Zebrafish neuromast hair cells are susceptible to cisplatin-induced hair cell cytotoxicity . Zebrafish larvae were treated with cisplatin (0.25 mM–1.5 mM) for four hours , fixed and the surviving GFP-labeled hair cells were imaged and counted. At 5 dpf, control zebrafish contained an average of 10.1±1.7 hair cells in the O2 neuromast. first orthopedics delawareWebFeb 15, 2024 · Cisplatin or doxorubicin induces pyroptosis of renal tubular epithelial cells It has been reported that the typical characteristics of pyroptosis were increased LDH release, increased PI-positive... first oriental grocery duluth