Flag-tag mouse monoclonal antibody

Author: ccyh

August undefined, 2024

WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. WebProduct Specific Information. This antibody detects Flag tag from various samples. similar epitope as the FLAG tag from Sigma. MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications. The MA1 … The membrane was probed with a DyLight 800-conjugated FLAG Epitope Tag … Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1 …

Flag-Tag Mouse Monoclonal Antibody ABP Biosciences

WebThe antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species … WebDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) DDDDK tag Monoclonal Antibody for ELISA. Host / Isotype. Mouse / IgG2b. Reactivity. human, mouse, rat . ... Monoclonal ANTI-FLAG? M2 … north curry

DYKDDDDK tag antibody (66008-4-Ig) Proteintech - ptglab

WebJun 15, 2012 · The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several highly specific monoclonal antibodies for FLAG are commercially available; however, they are not always sensitive enough to detect proteins expressed … WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived … WebHost Species Mouse; Clonality Monoclonal; Purification Affinity purification using immunogen. Applications WB,IF IP; Species Reactivity Hu Ms Rt; Specificity The Flag … north curry health centre prescriptions

DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight™ 488

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody …

WebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Standard Protocols Click here to view our Standard Protocols Publications All (610) WB … Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M how to resize an image in paint 3d by pixelWebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody how to resize an image in paint.net

"WebDetection was performed with ANTI-FLAG® M2 monoclonal primary antibody, anti-mouse-HRP secondary antibody, and ECL™ chemiluminescent substrate. Figure 3. Comparative Sensitivity of 3xFLAG®. Each protein fusion tag was cloned separately on the C terminal of GST. The purified proteins were diluted from 1 ug to 0.01 ng and analyzed. " - Flag-tag mouse monoclonal antibody

Flag-tag mouse monoclonal antibody

A high-affinity monoclonal antibody against the FLAG tag …

WebFlag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the … http://www.abpbiosciences.com/product/flag-tag/

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WebPolyclonal Antibody for studying Flag-Tag. Cited in 708 publications. Validated for WB, IP, F. Available in 2 sizes. ... (G3A1) mAb IgG1 Isotype Control #5415 for mouse monoclonal IgG1 primary antibodies, Mouse … WebMouse Antibody Monoclonal. Introduction. A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. …

WebFlag Tag Antibody is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, independently on N-terminal, C-terminal or internal regions of the target protein. Target Information WebHA-tag Rabbit Monoclonal Antibody staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. ... HA Tag Mouse Monoclonal Antibody. AG8050. 50μl. Flag Tag Rabbit …

WebKey features and details. Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization. Reacts with: … WebFLAG Tag Rabbit Polyclonal Antibody: 100μl: AF5051: Flag Tag Mouse Monoclonal Antibody: 50μl: AF519: Flag抗体(小鼠单抗) >40次: AF0033: Myc Tag Mouse Monoclonal Antibody: 100μl: AF5054: Myc Tag Mouse Monoclonal Antibody: 50μl: AF6513: c-Myc Rabbit Polyclonal Antibody: 50μl: AM926:

WebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ...

WebThe ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody … north curry post officeWebMonoclonal Antibody for studying Flag-Tag. Cited in 396 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse … north curry medical centreWebFlag Tag Monoclonal Antibody Datasheet Product Details Citations Customer Reviews and Q&A Product Details Citations Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2024 north curry churchWebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. how to resize an image in paint windows 10WebOct 4, 2024 · The FLAG tags are attached to the N-terminus or C-terminus of the target proteins. The tagged protein binds to the specific monoclonal antibodies conjugated on the matrix of magnetic beads or agarose gel. The residual impurities are washed off using the reagent washing buffer. north curry parish councilWebThe FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. how to resize an image in pygameWebJun 15, 2012 · The current study reports the successful establishment of hybridoma cells that produce an extremely high-affinity antibody to FLAG, namely 2H8 Ab. 2H8 Ab … how to resize an image in shotcut