Hipure total rna mini kit

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August undefined, 2024

WebTotal RNA was extracted using an RNeasy Mini Extraction kit (Qiagen) or the standard Trizol method. One microgram of RNA was reverse-transcribed in a total reaction volume of 20 ml containing 5 mM MgCl2, 106 buffer, 10 mM dithiothreitol, deoxyribonucleotide triphosphates, Poly-T primers, RNAsin 20 U, and 200 U of MuLV enzyme. WebMar 19, 2024 · Total RNA was isolated from leaves using a HiPure Plant RNA Mini Kit (Magen, Guangzhou, China). 1 μg of total RNA was used for synthesis of first-strand cDNA, using the PrimeScript RT reagent Kit with gDNA eraser (Takara Bio Inc., Otsu, Shiga, Japan). qPCR was conducted for the detection of gene transcripts in a Mini Option Real …

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WebJan 9, 2024 · Total RNA was extracted from cells using the HiPure Total RNA Mini Kit (Magen). Then, the RNA samples were sent for RNA-seq analysis to the Beijing Genomics Institute Tech Solutions Co. (Shenzhen ... WebBat capture, tissue collection and RNA extraction was conducted as previously reported (56) with the exception that RNAlater (Ambion) preserved spleen tissue from four male adult bats was pooled before tissue homogenisation and followed with total RNA extraction with the Qiagen RNeasy Mini kit with on-column extraction of genomic DNA with DNase ... timmy failure book one

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WebJan 5, 2024 · In brief, total RNA was extracted and purified using Magen Hipure Total RNA Mini Kit (Magen, Guangzhou, China), followed by constructing RNA sequencing libraries with KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems, Inc., MA, USA). After the construction of RNA sequencing libraries, Qubit 3.0 fluorometer (Invitrogen, CA, USA) … WebFind many great new & used options and get the best deals for MagMAX Total RNA Isolation Kit Applied Biosystems at the best online prices at eBay! Free shipping for many products! WebHiPure Universal RNA Kit R4130 Introduction Details Articles Download Contact us Introduction The Kit integrates phenol/guanidine-based lysis and silica membrane … timmy failure island poptropica walkthrough

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WebHiPure Universal RNA Kit R4130 Introduction Details Articles Download Contact us Introduction The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit … WebSep 15, 2024 · The HiPure Total RNA Mini Kit (Megen, Guangzhou, China) was used to extract total RNA from cells. The complementary DNA (cDNA) was synthesized using the TransScript One-Step gDNA Removal and Easyscript First Strand cDNA Synthesis SuperMix kits (TransGen BioTech, Beijing, China), following to the manufacturer's … timmy failure book 4WebRNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits. Figure 1. RNA yield from mouse brain tissue as determined by RNA … RNA was purified from 1 x 10 6 HeLa cells TRIzol Reagent, the ChargeSwitch Total … Dynabeads products center around the goals to provide consistently highest … The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic … The PureLink™ Pro 96 total RNA Purification Kit provides high-throughput … timmy failure book 7 release date

"WebThe Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase ... " - Hipure total rna mini kit

Hipure total rna mini kit

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WebAug 10, 2024 · Methods: Total RNA from tissues was isolated by Hipure Total RNA Mini Kit (Magen, Germany). Qubit 3.0 Fluorometer (Invitrogen, Carlsbad, California) was used for RNA concentration measurement while Agilent 2100 Bioanalyzer (Applied Biosystems, Carlsbad, CA) for RNA integrity estimation. A RIN value over 7.0 was considered eligible. WebThese three cell lines were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). We extracted total RNA from cells using the HiPure Total RNA Mini Kit (Magen, China) and then quantified the RNA with NanoDrop. ReverTra Ace qPCR RT Kit (Toyobo, China) was used to reverse transcribed the RNA …

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WebApr 10, 2024 · A Hipure Total RNA Mini kit (Magen, China) was used for the extraction of mRNA. And the standard qRT‐PCR experiment was performed according to our previous study . Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) was utilized as … WebMay 1, 2024 · Total RNA from cells was extracted and purified using HiPure Total RNA Mini Kit (Magen Cat# R4111-03). 1ug of extracted RNA was reverse-transcribed into cDNA using AB Script II RT master Mix (Abclonal Cat# RK20402). The RT-qPCR primers were listed in Table 2. Declaration of Competing Interest

WebDec 1, 2024 · The total RNA of P. chinensis and Arabidopsis was extracted from leaf tissues using the HiPure HP Plant RNA Mini Kit (Magen, China) following the manufacturer's … WebThe High Pure miRNA Isolation Kit purifies and enriches small RNAs, such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed, paraffin …

WebPurification Kits. For mini, midi, and maxi preparation of . plasmid DNA. Catalog Numbers K2100-02, K2100-03, K2100-04, K2100-05, K2100 06, and ... HiPure Mini and Midi … WebPurification Kits. For mini, midi, and maxi preparation of . plasmid DNA. Catalog Numbers K2100-02, K2100-03, K2100-04, K2100-05, K2100 06, and ... HiPure Mini and Midi Plasmid DNA Purification Kits are listed below. ... while RNA, proteins, carbohydrates and other impurities are washed away with Wash Buffer (W8). After washing, you ...

WebHipure Mirna Mini Column. filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit ... Supplier : Angen Biotech Co.,Ltd [China] [ 關聯分類 : Health & Medical, Examination & Testing Instrument, Medical Test …

WebHiPure Total RNA Kit IVD4121 Introduction Details Articles Download Contact us Introduction This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on. Details Specifications Principle timmy failure cast timmyWebThe HiPure total RNA Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug … timmy failure island walkthroughWebThe High Pure Viral RNA Kit is designed to purify viral RNA for RT-PCR analysis. Application The High Pure Viral RNA Kit is designed to purify intact viral RNA from serum or plasma samples. Viral RNA is used for RT-PCR … timmy failure islandWebHiPure Unviersal RNA Mini Kit通用型RNA提取试剂盒目录号：R4130-02 规格：50次价格：￥600 类别：总RNA提取试剂盒说明书下载产品介绍产品FAQs 已发表文献 HiPure … timmy failure island poptropica timmy failure castWebThe High Pure Viral RNA Kit . saves time, because the kit does not require extraction with organic solutions or nucleic acid precipitation and thus can prepare multiple RT-PCR … timmy failure island gameWebThe High Pure RNA Isolation Kit is designed for the purification of total RNA from cultured cells. Other sample materials like blood, yeast and bacteria require an additional specific … timmy failure: mistakes were made full movie