WebJul 23, 2010 · To assess the suitability of the NAPA promoter we made a translational fusion between the promoter fragment and the eGFP (encoding enhanced green fluorescent protein) and uidA [encoding β-glucuronidase (GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. … WebThis intermediate entry vector was recombined with the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants were selected on Gamborg's B5 plates containing hygromycin (50 µg/liter).
Supplementary Materials for - Science
WebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). WebAug 1, 2010 · GATEWAY-compatible destination vectors (Karimi et al. 2002) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from … sand rakes for beaches
Amplification, cloning and confirmation of the Adh promoter from ...
WebMar 14, 2024 · Download. Get Updates Share This. Summary. Files. Reviews. Support. Code. OpenPFGW is software that is designed to perform PRP and primality tests on numbers of … Web(GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. GUS activity was observed in develop-ing … WebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … shoreline family chiropractic west haven