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Phgwfs7.0

WebJul 23, 2010 · To assess the suitability of the NAPA promoter we made a translational fusion between the promoter fragment and the eGFP (encoding enhanced green fluorescent protein) and uidA [encoding β-glucuronidase (GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. … WebThis intermediate entry vector was recombined with the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants were selected on Gamborg's B5 plates containing hygromycin (50 µg/liter).

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WebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). WebAug 1, 2010 · GATEWAY-compatible destination vectors (Karimi et al. 2002) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from … sand rakes for beaches https://sac1st.com

Amplification, cloning and confirmation of the Adh promoter from ...

WebMar 14, 2024 · Download. Get Updates Share This. Summary. Files. Reviews. Support. Code. OpenPFGW is software that is designed to perform PRP and primality tests on numbers of … Web(GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. GUS activity was observed in develop-ing … WebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … shoreline family chiropractic west haven

Vectors pHGWFS7, pH7GW2 and pENTR/D, respectively.

Category:A chloroplast envelope membrane protein containing a putative …

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Phgwfs7.0

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WebMar 24, 2024 · We prepared genomic fragments (1) from 4.2 kbp upstream of the translation start site of HAM1 (AT2G45160), which contains the promoter region, to the TS of miR171a; (2) from the TS to the 3′-UTR end; and (3) from the linearized vector (from pHGWFS7.0) by PCR using primer nos. 7 and 8, 9 and 10, and 11 and 12, respectively (see Supplemental ... Webwith the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants …

Phgwfs7.0

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WebFeb 28, 2024 · In cold-primed plants, the pleiotropically stress regulated genes CHS (chalcone synthase; At5g13930) and PAL1 (phenylalanine ammonium lyase; At2g37040) were stronger activated by a second cold... WebThe destination vector is pHGWFS7,0 (13 kb). The donor vector is pDONR221. I attached reference protocols for single-step BP/LR gateway. Any comment and advice will be really …

WebEnter your name and contact information. To better ensure the security of donor accounts, we need to prove you are a human. We apologize for the inconvenience. Your donation is … WebNdeI for a single cut fragment, L7:1kb + DNA ladder, L1-L6: Digested samples, L2: Positive sample with true single cut. 1E: Plasmid isolation from pHGWFS7.0_Adh vector.

WebNWCG WebGATEWAY -compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), pro- moter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium).

WebVIB-UGent Center for Plant Systems Biology. Gateway Vectors. Collection. pKGWFS7.

Web(GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. GUS activity was observed in develop-ing seeds in several independent transformants, but was absent from other tissues and organs including pollen. GUS activity was reproducibly detected in embryos from late shoreline family medical clinicWebCCATTAACAAATCTCCG-30; and a T-DNA insertion primer, LBb1.3, 5 0-ATTTTGCCGATTTCGGAAC-3 ). The zfp5 gis3, ... RNAi construct, whereas pHGWFS7 was used for the pGIS3:GUS construct. For all cloning constructs, targeted gene-specific fragments were first PCR-amplified from cDNA sand rake machineWebLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors sandra k. luedke 63 of flower moundWeb*Photo may vary from actual product. This product has been confirmed that it does not contain the 6 substances specified in EU RoHS Directive 2011/65/EU and the 4 … shoreline family health care branfordWebwere sterilized with 0.3% Sodium Hypochlorite and pre-germinated on 0.9% bactoagar plates at 30 C. Seven day old seedlings were transferred to sterilized sand and grown for two weeks without fertilizer. Plantlets were then transferred to liquid ½ Hoagland containing 25 M phosphate for another week before treating them either with GSE, sandra kennedy arizona corporation commissionWebFeb 28, 2024 · Arabidopsis thaliana var. Col-0 wildtype plants and transgenic lines were grown on ... Carlsbad, U.S.A.) into the Gateway site of the vector pHGWFS7.0 101 … sandra kinder psychotherapeutinWebLOCUS pHGWFS7 13124 bp DNA circular 23-FEB-2024 DEFINITION . ACCESSION urn.local...1q-dnt3ece KEYWORDS Promoter analysis. shoreline family practice guilford ct